Chick embryo ribosome crystals: analysis of bonding and functional activity in vitro.

نویسنده

  • B Byers
چکیده

The intracellular ribosome crystals from chick embryos incubated at 5 degrees C have been isolated; stable products of their dissociation were shown to include single ribosomes, tetramers of ribosomes, and tetramers of large subunits. Conditions were found for the formation in vitro not only of the p4 crystals from ribosomal tetramers but also of the tetramers themselves from free ribosomes. The polypeptide synthesizing activity of these ribosomes was found to be unimpaired by their constraint in the tetrameric configuration.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A Chick Embryo in-Vitro Model of Knee Morphogenesis

   Background: In this feasibility study, a mechanically loaded in-vitro tissue culture model of joint morphogenesis using the isolated lower extremity of the 8 day old chick embryo was developed to assess the effects of mechanical loading on joint morphogenesis. Methods: The developed in-vitro system allows controlled flexion and extension of the chick embryonic knee with a range of motion of ...

متن کامل

Anti- Angiogenic Activity of Vitex agnus castus Methanol Extract in vivo Study

This study aimed to investigate the anti-angiogenic activity of Vitex agnus castus methanol extract in vivo. Eggs were incubated for three days, small whole made on the fine pinpoint, next day the egg’s sac penetrated and a small frame was made in the shell. The window was resealed and eggs were returned to the incubator until day 10 of chick embryo development, 20 µl of 500mg/ml of the methano...

متن کامل

Ribosome crystals in necrotizing cells from the posterior necrotic zone of the developing chick limb.

Ribosome and ' protein crystals' were observed in electron micrographs of degenerating cells from the posterior necrotic zone (PNZ) of developing chick limbs, stages 22 through 24. Ribosome crystals were made up of basic units of 4 ribosomes arranged in a square tetramer. They were monomorphic with the surface lattice being of the p4 plane group. The i-ribosomethick sheets of crystals were ofte...

متن کامل

Effect of Adenosine Agonists on the Proliferation and Differentiation of Chick Embryo Fibroblasts in Three Dimensional Reconstituted Tissue Constructs

Previous studies indicate that organ fibroblasts play an important role in wound healing, collagen production, remodeling processes and pathogenesis of progressive heart, lung, renal and hepatic fibrotic diseases. Several studies suggest a possible inhibitory role for adenosine in the regulation of fibroblast proliferation. The effect of adenosine A2 agonists on proliferation and differentiatio...

متن کامل

In vitro crystallization of ribosomes from chick embryos

A new two-dimensional ribosome crystal, having the tetragonal space group P42(1)2 (a = 593 A), has been grown from ribosome tetramers extracted from hypothermic chick embryos. It is of particular interest because of its larger size (up to 3 x 3 micron2) and greater stability compared to other related polymorphic forms, and because it can easily be grown in large amounts. X-ray diffraction shows...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 68 2  شماره 

صفحات  -

تاریخ انتشار 1971